Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24881 | 3' | -46.9 | NC_005284.1 | + | 26595 | 0.66 | 0.99275 |
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Target: 5'- ---gUCGAGCAGGuAUUCGCAucucauCGGGa -3' miRNA: 3'- gaagAGCUUGUUCuUAAGCGU------GCCUc -5' |
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| 24881 | 3' | -46.9 | NC_005284.1 | + | 18457 | 0.67 | 0.986976 |
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Target: 5'- -aUCUaucaGGGCAAGuaccUCGCGCGGGu -3' miRNA: 3'- gaAGAg---CUUGUUCuua-AGCGUGCCUc -5' |
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| 24881 | 3' | -46.9 | NC_005284.1 | + | 10578 | 0.67 | 0.986976 |
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Target: 5'- --cCUCGGcgucgACGAGAAaUCGCGCGaGAu -3' miRNA: 3'- gaaGAGCU-----UGUUCUUaAGCGUGC-CUc -5' |
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| 24881 | 3' | -46.9 | NC_005284.1 | + | 40871 | 0.67 | 0.983003 |
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Target: 5'- ---aUCGGAUugccGAGUUCaGCGCGGAGc -3' miRNA: 3'- gaagAGCUUGuu--CUUAAG-CGUGCCUC- -5' |
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| 24881 | 3' | -46.9 | NC_005284.1 | + | 5306 | 0.69 | 0.94866 |
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Target: 5'- cCUUCUCGAugGcggcucAGAucuucgacCGCAUGGAGa -3' miRNA: 3'- -GAAGAGCUugU------UCUuaa-----GCGUGCCUC- -5' |
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| 24881 | 3' | -46.9 | NC_005284.1 | + | 30721 | 0.7 | 0.932818 |
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Target: 5'- aUUUUCGAu--GGAAUguaGCACGGGGg -3' miRNA: 3'- gAAGAGCUuguUCUUAag-CGUGCCUC- -5' |
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| 24881 | 3' | -46.9 | NC_005284.1 | + | 47288 | 0.72 | 0.877096 |
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Target: 5'- ---gUCGAcCGAGAAUUCGCcCGGAu -3' miRNA: 3'- gaagAGCUuGUUCUUAAGCGuGCCUc -5' |
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| 24881 | 3' | -46.9 | NC_005284.1 | + | 9070 | 0.75 | 0.717319 |
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Target: 5'- --gCUUGGACAAGcGUUCGCgGCGGGGc -3' miRNA: 3'- gaaGAGCUUGUUCuUAAGCG-UGCCUC- -5' |
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| 24881 | 3' | -46.9 | NC_005284.1 | + | 13879 | 1.11 | 0.00599 |
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Target: 5'- cCUUCUCGAACAAGAAUUCGCACGGAGa -3' miRNA: 3'- -GAAGAGCUUGUUCUUAAGCGUGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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