Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24881 | 5' | -56.2 | NC_005284.1 | + | 12098 | 0.69 | 0.486523 |
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Target: 5'- gGCUCCGCuuugGGAUUCGGCGGugucUCUGa -3' miRNA: 3'- aCGAGGCGcga-CUUGAGCCGUU----AGGC- -5' |
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| 24881 | 5' | -56.2 | NC_005284.1 | + | 44897 | 0.69 | 0.44652 |
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Target: 5'- cGCUUcggCGCGCUGAGCUccugcugugCGGCug-CCGu -3' miRNA: 3'- aCGAG---GCGCGACUUGA---------GCCGuuaGGC- -5' |
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| 24881 | 5' | -56.2 | NC_005284.1 | + | 35453 | 0.7 | 0.417753 |
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Target: 5'- uUGUUCguCGCGCUGGAuCUCGcGCGGUgCGa -3' miRNA: 3'- -ACGAG--GCGCGACUU-GAGC-CGUUAgGC- -5' |
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| 24881 | 5' | -56.2 | NC_005284.1 | + | 39128 | 0.71 | 0.363753 |
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Target: 5'- cGC-CCGCGU--GAUUCGGCAcgCCGu -3' miRNA: 3'- aCGaGGCGCGacUUGAGCCGUuaGGC- -5' |
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| 24881 | 5' | -56.2 | NC_005284.1 | + | 31759 | 0.71 | 0.34686 |
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Target: 5'- cGCUCUGCGCaGAGCUUGuCGucAUCCGu -3' miRNA: 3'- aCGAGGCGCGaCUUGAGCcGU--UAGGC- -5' |
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| 24881 | 5' | -56.2 | NC_005284.1 | + | 14626 | 0.72 | 0.298121 |
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Target: 5'- cGCUCgCGCGCuaccucgacgccgUGAAUUuuccggcCGGCAAUCCGa -3' miRNA: 3'- aCGAG-GCGCG-------------ACUUGA-------GCCGUUAGGC- -5' |
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| 24881 | 5' | -56.2 | NC_005284.1 | + | 14572 | 0.74 | 0.238309 |
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Target: 5'- cGCUgUGCGUUGcGCUUGGCGAUCuCGu -3' miRNA: 3'- aCGAgGCGCGACuUGAGCCGUUAG-GC- -5' |
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| 24881 | 5' | -56.2 | NC_005284.1 | + | 8244 | 0.76 | 0.178007 |
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Target: 5'- cGCU-CGCGCUGcGGCUCGGCAAgacguuggCCGa -3' miRNA: 3'- aCGAgGCGCGAC-UUGAGCCGUUa-------GGC- -5' |
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| 24881 | 5' | -56.2 | NC_005284.1 | + | 13914 | 1.09 | 0.000716 |
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Target: 5'- aUGCUCCGCGCUGAACUCGGCAAUCCGa -3' miRNA: 3'- -ACGAGGCGCGACUUGAGCCGUUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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