Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24884 | 3' | -60.2 | NC_005284.1 | + | 10001 | 0.69 | 0.340103 |
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Target: 5'- cGCuGGCCGagacggaCCGCGGCCUUgCgcaagcCGAGCg -3' miRNA: 3'- -CGuCCGGCa------GGCGCUGGAG-Ga-----GCUCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 9587 | 0.69 | 0.324628 |
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Target: 5'- --cGuGCCGUUCGacaCGGCCgUCUUCGAGCg -3' miRNA: 3'- cguC-CGGCAGGC---GCUGG-AGGAGCUCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 44389 | 0.7 | 0.281381 |
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Target: 5'- --cGGCCGcCCGCGacGCCUCUugcuUCGuGCg -3' miRNA: 3'- cguCCGGCaGGCGC--UGGAGG----AGCuCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 35223 | 0.71 | 0.25517 |
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Target: 5'- uGCGGGCCGUCUucgGUGuCgUagaUCUCGAGCa -3' miRNA: 3'- -CGUCCGGCAGG---CGCuGgA---GGAGCUCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 23663 | 0.71 | 0.236852 |
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Target: 5'- -gAGGCCGaCCGCGACaUCC-CGaAGCu -3' miRNA: 3'- cgUCCGGCaGGCGCUGgAGGaGC-UCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 33599 | 0.72 | 0.198381 |
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Target: 5'- uCAGGaCCGgcUCCGCGugCUCgaugagccguugCUCGAGCc -3' miRNA: 3'- cGUCC-GGC--AGGCGCugGAG------------GAGCUCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 11815 | 0.72 | 0.198381 |
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Target: 5'- aGCAGGUCGcgcgcgagCGCGGCUaUCUCGAGCa -3' miRNA: 3'- -CGUCCGGCag------GCGCUGGaGGAGCUCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 39581 | 0.73 | 0.183629 |
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Target: 5'- --cGGCCG-CUGCGuaguCCUCCgacgCGAGCg -3' miRNA: 3'- cguCCGGCaGGCGCu---GGAGGa---GCUCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 42100 | 0.77 | 0.089266 |
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Target: 5'- uCAGGCCGUCCGCGGCCcgCUgggUGuAGCc -3' miRNA: 3'- cGUCCGGCAGGCGCUGGa-GGa--GC-UCG- -5' |
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| 24884 | 3' | -60.2 | NC_005284.1 | + | 11509 | 1.12 | 0.000213 |
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Target: 5'- aGCAGGCCGUCCGCGACCUCCUCGAGCu -3' miRNA: 3'- -CGUCCGGCAGGCGCUGGAGGAGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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