Results 81 - 83 of 83 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24887 | 3' | -51 | NC_005284.1 | + | 50126 | 0.69 | 0.813225 |
Target: 5'- uGACGGUcCGCGACGaCCaGCGAGUu--- -3' miRNA: 3'- gCUGUUA-GCGCUGC-GG-CGCUUAuagc -5' |
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24887 | 3' | -51 | NC_005284.1 | + | 50664 | 0.72 | 0.665936 |
Target: 5'- aGAC-AUCGCgGACGUgGCGGAauUAUCGu -3' miRNA: 3'- gCUGuUAGCG-CUGCGgCGCUU--AUAGC- -5' |
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24887 | 3' | -51 | NC_005284.1 | + | 54639 | 0.66 | 0.929124 |
Target: 5'- aCGACGAgaaccugcaaGCGAucUGCCGUGAcUGUCa -3' miRNA: 3'- -GCUGUUag--------CGCU--GCGGCGCUuAUAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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