miRNA: 3'- -GCUGUuaGCGcUGCGG--CGCUUA---UAGC- -5'
miRNA: 3'- -GCUGUUaGCGCUGCGG----CGCUuAU--AGC- -5'
miRNA: 3'- -GCUG-UUAG-CGCU-GCGGCGCUUaUAGC- -5'
Results 81 - 83 of 83 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24887 | 3' | -51 | NC_005284.1 | + | 3566 | 0.69 | 0.831866 |
|
Target: 5'- aCGACAcgUGCaGCGCUugGCGAGUgcuAUCGa -3' miRNA: 3'- -GCUGUuaGCGcUGCGG--CGCUUA---UAGC- -5' |
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| 24887 | 3' | -51 | NC_005284.1 | + | 1790 | 0.7 | 0.752954 |
|
Target: 5'- gCGGCAAaCGCGAUGCCcaccGCGAuUGccUCGg -3' miRNA: 3'- -GCUGUUaGCGCUGCGG----CGCUuAU--AGC- -5' |
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| 24887 | 3' | -51 | NC_005284.1 | + | 893 | 0.66 | 0.917152 |
|
Target: 5'- aCGGCaAAUCgGUGAuUGCCGCGGGcGUUGg -3' miRNA: 3'- -GCUG-UUAG-CGCU-GCGGCGCUUaUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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