Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24889 | 3' | -50.5 | NC_005284.1 | + | 19387 | 0.69 | 0.772239 |
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Target: 5'- gAUUCGCCGUgaucgacgaggGCGGUGCAGGcgugAUCGGCg -3' miRNA: 3'- gUAGGCGGUA-----------CGCUAUGUCU----UGGUUG- -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 18254 | 0.69 | 0.76164 |
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Target: 5'- -uUCCGCCAU-CGGUGCAGccGCCGc- -3' miRNA: 3'- guAGGCGGUAcGCUAUGUCu-UGGUug -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 928 | 0.7 | 0.72903 |
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Target: 5'- --gCCGCUGUGCucGAUGCuGAACUGGCg -3' miRNA: 3'- guaGGCGGUACG--CUAUGuCUUGGUUG- -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 25577 | 0.71 | 0.684133 |
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Target: 5'- --cCCGCCGUccGCGcgGCGGuuGCCGACu -3' miRNA: 3'- guaGGCGGUA--CGCuaUGUCu-UGGUUG- -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 40369 | 0.72 | 0.638365 |
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Target: 5'- gAUCCGCCGgaGCGGUGCAGcGAgCAAg -3' miRNA: 3'- gUAGGCGGUa-CGCUAUGUC-UUgGUUg -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 33293 | 0.72 | 0.638365 |
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Target: 5'- --gCCGCCAUGCcaaGCAGcgcGGCCAACa -3' miRNA: 3'- guaGGCGGUACGcuaUGUC---UUGGUUG- -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 62 | 0.73 | 0.569734 |
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Target: 5'- gCAUCCGCCggGCGGgcaacgACGGAcggGCCAc- -3' miRNA: 3'- -GUAGGCGGuaCGCUa-----UGUCU---UGGUug -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 1669 | 0.73 | 0.569734 |
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Target: 5'- -uUUCGCCGUGCGAgGCGuGAugUAGCg -3' miRNA: 3'- guAGGCGGUACGCUaUGU-CUugGUUG- -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 31009 | 0.73 | 0.558432 |
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Target: 5'- --gCCGCCGUGCG-UGCGGAaguuGCCGGu -3' miRNA: 3'- guaGGCGGUACGCuAUGUCU----UGGUUg -5' |
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| 24889 | 3' | -50.5 | NC_005284.1 | + | 8481 | 1.11 | 0.002034 |
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Target: 5'- cCAUCCGCCAUGCGAUACAGAACCAACg -3' miRNA: 3'- -GUAGGCGGUACGCUAUGUCUUGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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