Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24890 | 5' | -57 | NC_005284.1 | + | 44894 | 0.66 | 0.664085 |
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Target: 5'- -cGACGcUUCGGCGcgcugaGCUCcuGCUGUGCg -3' miRNA: 3'- ccCUGC-AAGCCGCaa----CGGG--UGACGCG- -5' |
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| 24890 | 5' | -57 | NC_005284.1 | + | 44953 | 0.68 | 0.504816 |
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Target: 5'- --cGCGUagGGCG-UGCCCGugaUGCGCg -3' miRNA: 3'- cccUGCAagCCGCaACGGGUg--ACGCG- -5' |
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| 24890 | 5' | -57 | NC_005284.1 | + | 47072 | 0.66 | 0.653332 |
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Target: 5'- cGGcGGCGgcacgagcUUCGGCGcaccGCUCAC-GCGCa -3' miRNA: 3'- -CC-CUGC--------AAGCCGCaa--CGGGUGaCGCG- -5' |
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| 24890 | 5' | -57 | NC_005284.1 | + | 47505 | 0.69 | 0.452122 |
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Target: 5'- aGGAugcuaucgccgucgUGUUCGGCGUcgaggUGCCgACcgUGCGCu -3' miRNA: 3'- cCCU--------------GCAAGCCGCA-----ACGGgUG--ACGCG- -5' |
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| 24890 | 5' | -57 | NC_005284.1 | + | 50330 | 0.68 | 0.51506 |
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Target: 5'- uGGcGGCGUgccgCGGCGaaccgUGCUUuCUGCGUg -3' miRNA: 3'- -CC-CUGCAa---GCCGCa----ACGGGuGACGCG- -5' |
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| 24890 | 5' | -57 | NC_005284.1 | + | 53886 | 0.74 | 0.24608 |
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Target: 5'- cGGGAUGggCGGcCGUcgucgcuUGCCUuggcCUGCGCg -3' miRNA: 3'- -CCCUGCaaGCC-GCA-------ACGGGu---GACGCG- -5' |
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| 24890 | 5' | -57 | NC_005284.1 | + | 54704 | 0.69 | 0.455026 |
|
Target: 5'- cGGuGACGUgguggUGGCccguccgucGUUGCCCGCccggcggaUGCGCc -3' miRNA: 3'- -CC-CUGCAa----GCCG---------CAACGGGUG--------ACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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