Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24895 | 5' | -54.3 | NC_005284.1 | + | 31619 | 0.66 | 0.740703 |
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Target: 5'- gGGUgCGGGCGGCgUCGA--GggCGCAu -3' miRNA: 3'- gUCA-GCCCGUCG-AGCUaaCuaGCGUc -5' |
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| 24895 | 5' | -54.3 | NC_005284.1 | + | 10405 | 0.66 | 0.730051 |
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Target: 5'- gAGgcgucgCGGGCGGC-CGAU-GAcgCGCAGu -3' miRNA: 3'- gUCa-----GCCCGUCGaGCUAaCUa-GCGUC- -5' |
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| 24895 | 5' | -54.3 | NC_005284.1 | + | 12298 | 0.7 | 0.536305 |
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Target: 5'- gCAGcCGGGCGuGCgcgcacaccuugagCGAUUGAaCGCAGg -3' miRNA: 3'- -GUCaGCCCGU-CGa-------------GCUAACUaGCGUC- -5' |
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| 24895 | 5' | -54.3 | NC_005284.1 | + | 54620 | 0.72 | 0.42931 |
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Target: 5'- cCAGUCcGGCGGCUCGAUgu-UCGUg- -3' miRNA: 3'- -GUCAGcCCGUCGAGCUAacuAGCGuc -5' |
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| 24895 | 5' | -54.3 | NC_005284.1 | + | 22928 | 0.72 | 0.382516 |
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Target: 5'- cCAGUCGGGCuGUucugaUCGAUUGG-CGCGc -3' miRNA: 3'- -GUCAGCCCGuCG-----AGCUAACUaGCGUc -5' |
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| 24895 | 5' | -54.3 | NC_005284.1 | + | 43270 | 0.79 | 0.141484 |
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Target: 5'- ---aUGGGCAGCUCGAggaGGUCGCGGa -3' miRNA: 3'- gucaGCCCGUCGAGCUaa-CUAGCGUC- -5' |
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| 24895 | 5' | -54.3 | NC_005284.1 | + | 5991 | 1.08 | 0.001354 |
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Target: 5'- cCAGUCGGGCAGCUCGAUUGAUCGCAGu -3' miRNA: 3'- -GUCAGCCCGUCGAGCUAACUAGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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