Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24897 | 3' | -59 | NC_005284.1 | + | 48043 | 0.67 | 0.439315 |
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Target: 5'- -cCCUUCGCACGUuggaggaggCCgGUGCGCUGc- -3' miRNA: 3'- uaGGGGGCGUGCA---------GGgUACGCGAUag -5' |
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| 24897 | 3' | -59 | NC_005284.1 | + | 46858 | 0.67 | 0.439315 |
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Target: 5'- -aCCCCgGCgAUGUCCCGaaGCGCU-UCg -3' miRNA: 3'- uaGGGGgCG-UGCAGGGUa-CGCGAuAG- -5' |
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| 24897 | 3' | -59 | NC_005284.1 | + | 45418 | 0.67 | 0.420355 |
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Target: 5'- gAUCCCCUGCGCaagCCUc-GCGCUcgCg -3' miRNA: 3'- -UAGGGGGCGUGca-GGGuaCGCGAuaG- -5' |
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| 24897 | 3' | -59 | NC_005284.1 | + | 9624 | 0.67 | 0.392864 |
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Target: 5'- gAUCUCCUGCccgACGUCgCCGUGCGUc--- -3' miRNA: 3'- -UAGGGGGCG---UGCAG-GGUACGCGauag -5' |
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| 24897 | 3' | -59 | NC_005284.1 | + | 40329 | 0.68 | 0.358077 |
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Target: 5'- cUCCCuuGCGgGUCCCGguaCGCUGg- -3' miRNA: 3'- uAGGGggCGUgCAGGGUac-GCGAUag -5' |
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| 24897 | 3' | -59 | NC_005284.1 | + | 24941 | 0.69 | 0.325505 |
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Target: 5'- gAUCCCuCCGCGCcgaUCAUGCGCUccgcGUCu -3' miRNA: 3'- -UAGGG-GGCGUGcagGGUACGCGA----UAG- -5' |
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| 24897 | 3' | -59 | NC_005284.1 | + | 8273 | 0.69 | 0.302555 |
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Target: 5'- cUCCCuUCGCAccaucCGUCCCGUGCGgcGUCg -3' miRNA: 3'- uAGGG-GGCGU-----GCAGGGUACGCgaUAG- -5' |
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| 24897 | 3' | -59 | NC_005284.1 | + | 31142 | 0.71 | 0.241237 |
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Target: 5'- gAUCgCCCCGUACaGUgCCCGUGaCGCgGUCg -3' miRNA: 3'- -UAG-GGGGCGUG-CA-GGGUAC-GCGaUAG- -5' |
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| 24897 | 3' | -59 | NC_005284.1 | + | 3862 | 1.07 | 0.00052 |
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Target: 5'- cAUCCCCCGCACGUCCCAUGCGCUAUCu -3' miRNA: 3'- -UAGGGGGCGUGCAGGGUACGCGAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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