Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24898 | 3' | -49.3 | NC_005284.1 | + | 39699 | 0.66 | 0.967426 |
Target: 5'- -gCGCcgCGAGGcAGACgGCGcagUUCGAg -3' miRNA: 3'- gaGCGaaGCUCC-UUUGgCGUa--AAGCU- -5' |
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24898 | 3' | -49.3 | NC_005284.1 | + | 45006 | 0.68 | 0.918676 |
Target: 5'- -aCGCUUC-AGGAAGCggugCGCAUUggCGAg -3' miRNA: 3'- gaGCGAAGcUCCUUUG----GCGUAAa-GCU- -5' |
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24898 | 3' | -49.3 | NC_005284.1 | + | 19257 | 0.68 | 0.918676 |
Target: 5'- cCUCGCgaccgCGAcacGGGAAUCGCucUUCGAc -3' miRNA: 3'- -GAGCGaa---GCU---CCUUUGGCGuaAAGCU- -5' |
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24898 | 3' | -49.3 | NC_005284.1 | + | 39912 | 0.68 | 0.890947 |
Target: 5'- -gCGCUgacUCGAGGucggcuGCUGCGuUUUCGAg -3' miRNA: 3'- gaGCGA---AGCUCCuu----UGGCGU-AAAGCU- -5' |
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24898 | 3' | -49.3 | NC_005284.1 | + | 10654 | 0.69 | 0.849971 |
Target: 5'- -gCGCUgcgCGAGGAAcuggugcgcGCCGCGggcgCGAu -3' miRNA: 3'- gaGCGAa--GCUCCUU---------UGGCGUaaa-GCU- -5' |
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24898 | 3' | -49.3 | NC_005284.1 | + | 3685 | 1.1 | 0.003777 |
Target: 5'- cCUCGCUUCGAGGAAACCGCAUUUCGAu -3' miRNA: 3'- -GAGCGAAGCUCCUUUGGCGUAAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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