miRNA: 3'- gCG-CUugCUGCGGCUCuacGUCCGCC- -5'
miRNA: 3'- gC-GCUUGCUG---CG----GCUc-UACGUCCGCC- -5'
miRNA: 3'- gCGCU--UGCUGCG-GCUC-------------UACguCCGCC- -5'
miRNA: 3'- gCGCUUGCuGCGGCUCua-CgUCCgCC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24907 | 5' | -57.2 | NC_005284.1 | + | 51460 | 0.69 | 0.459834 |
|
Target: 5'- aGCuGAugGACGUCGAGcgaUGGGCGGu -3' miRNA: 3'- gCG-CUugCUGCGGCUCuacGUCCGCC- -5' |
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| 24907 | 5' | -57.2 | NC_005284.1 | + | 53638 | 0.66 | 0.614655 |
|
Target: 5'- aGaCGAGCGACauuGCguuaCGAcuGUGCAGGCGGc -3' miRNA: 3'- gC-GCUUGCUG---CG----GCUc-UACGUCCGCC- -5' |
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| 24907 | 5' | -57.2 | NC_005284.1 | + | 54679 | 0.72 | 0.335239 |
|
Target: 5'- gGCGAagACGGCGCgCGAGcgguugcggugacGUGguGGUGGc -3' miRNA: 3'- gCGCU--UGCUGCG-GCUC-------------UACguCCGCC- -5' |
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| 24907 | 5' | -57.2 | NC_005284.1 | + | 54742 | 0.7 | 0.386014 |
|
Target: 5'- gGCGGAUG-CGCCGGGuggGgAGGgGGg -3' miRNA: 3'- gCGCUUGCuGCGGCUCua-CgUCCgCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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