Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24907 | 5' | -57.2 | NC_005284.1 | + | 5952 | 0.71 | 0.34726 |
Target: 5'- uGCGGACGccacggcugGCGCCGAuccgaucgucgcgacGAUGCgauucGGGCGGc -3' miRNA: 3'- gCGCUUGC---------UGCGGCU---------------CUACG-----UCCGCC- -5' |
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24907 | 5' | -57.2 | NC_005284.1 | + | 5921 | 1.1 | 0.000672 |
Target: 5'- aCGCGAACGACGCCGAGAUGCAGGCGGc -3' miRNA: 3'- -GCGCUUGCUGCGGCUCUACGUCCGCC- -5' |
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24907 | 5' | -57.2 | NC_005284.1 | + | 4011 | 0.67 | 0.599678 |
Target: 5'- gCGCGAAuCGACGCgGuGAaaucgaucaucgcGCAcGGCGGa -3' miRNA: 3'- -GCGCUU-GCUGCGgCuCUa------------CGU-CCGCC- -5' |
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24907 | 5' | -57.2 | NC_005284.1 | + | 938 | 0.68 | 0.519812 |
Target: 5'- uGCuGGACGACgaguucgguGCCGAGGUGUAcgccGGCGc -3' miRNA: 3'- gCG-CUUGCUG---------CGGCUCUACGU----CCGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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