Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24909 | 3' | -54.2 | NC_005284.1 | + | 16763 | 0.7 | 0.548678 |
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Target: 5'- gCGCGugCGCuUCGgUGUGccGGCGCUa -3' miRNA: 3'- -GUGUugGCGuAGCaGCACuuCCGCGA- -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 49294 | 0.73 | 0.387738 |
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Target: 5'- uCACGGCCGC-UCGUCGUucccGAGGGUacgGCg -3' miRNA: 3'- -GUGUUGGCGuAGCAGCA----CUUCCG---CGa -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 37449 | 0.73 | 0.387738 |
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Target: 5'- gACGACCGCggCGUCaacgucgaaGUGAAGGgGCc -3' miRNA: 3'- gUGUUGGCGuaGCAG---------CACUUCCgCGa -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 19344 | 0.73 | 0.378732 |
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Target: 5'- gACAACgGCAUCGUCGaaucGcAGGUGCUu -3' miRNA: 3'- gUGUUGgCGUAGCAGCa---CuUCCGCGA- -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 50087 | 0.74 | 0.335852 |
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Target: 5'- aGCAGCgGUA-CGUCGUG-GGGCGCg -3' miRNA: 3'- gUGUUGgCGUaGCAGCACuUCCGCGa -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 45778 | 0.83 | 0.088906 |
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Target: 5'- gUACAGCCGUGUCGacgcgcggUCGUGAAGGCGUg -3' miRNA: 3'- -GUGUUGGCGUAGC--------AGCACUUCCGCGa -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 8135 | 1.07 | 0.001777 |
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Target: 5'- gCACAACCGCAUCGUCGUGAAGGCGCUc -3' miRNA: 3'- -GUGUUGGCGUAGCAGCACUUCCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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