miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
24909 3' -54.2 NC_005284.1 + 18764 0.67 0.703138
Target:  5'- -uUggUCGCGUCGUCGcGAuucGGCGUg -3'
miRNA:   3'- guGuuGGCGUAGCAGCaCUu--CCGCGa -5'
24909 3' -54.2 NC_005284.1 + 11276 0.67 0.713995
Target:  5'- gGCGACCGUcUCGUC--GAAGGCuuGCUu -3'
miRNA:   3'- gUGUUGGCGuAGCAGcaCUUCCG--CGA- -5'
24909 3' -54.2 NC_005284.1 + 22982 0.67 0.721546
Target:  5'- gGCugcGCCGCAUCGUCccaacUGAAaaugaucagcucgcGGCGCUc -3'
miRNA:   3'- gUGu--UGGCGUAGCAGc----ACUU--------------CCGCGA- -5'
24909 3' -54.2 NC_005284.1 + 21955 0.66 0.746023
Target:  5'- cCGCAACCGCugCGUCGacgucUGAuAGGCGg- -3'
miRNA:   3'- -GUGUUGGCGuaGCAGC-----ACU-UCCGCga -5'
24909 3' -54.2 NC_005284.1 + 9080 0.66 0.786029
Target:  5'- aCGCGACCGCcuugcugAUCGUgGUGAcaagcGGGC-Cg -3'
miRNA:   3'- -GUGUUGGCG-------UAGCAgCACU-----UCCGcGa -5'
24909 3' -54.2 NC_005284.1 + 5380 0.66 0.787025
Target:  5'- uCugAGCCGcCAUCGa---GAAGGCGCc -3'
miRNA:   3'- -GugUUGGC-GUAGCagcaCUUCCGCGa -5'
24909 3' -54.2 NC_005284.1 + 45778 0.83 0.088906
Target:  5'- gUACAGCCGUGUCGacgcgcggUCGUGAAGGCGUg -3'
miRNA:   3'- -GUGUUGGCGUAGC--------AGCACUUCCGCGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.