Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24909 | 3' | -54.2 | NC_005284.1 | + | 33359 | 0.68 | 0.659121 |
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Target: 5'- aACGacACCGCccguUCGUCG-GccGGCGCUg -3' miRNA: 3'- gUGU--UGGCGu---AGCAGCaCuuCCGCGA- -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 11343 | 0.68 | 0.659121 |
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Target: 5'- gCGCAACUGCG-CGUCGaGgcGGuCGCa -3' miRNA: 3'- -GUGUUGGCGUaGCAGCaCuuCC-GCGa -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 15297 | 0.68 | 0.659121 |
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Target: 5'- gAgGACCGCA-CGaUGUGcGGGCGCa -3' miRNA: 3'- gUgUUGGCGUaGCaGCACuUCCGCGa -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 51748 | 0.67 | 0.681225 |
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Target: 5'- uCGCGGCgCGCcugCGggaaGUGGAGGCGUUa -3' miRNA: 3'- -GUGUUG-GCGua-GCag--CACUUCCGCGA- -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 8102 | 0.67 | 0.692211 |
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Target: 5'- uGCAACCGCG--GUCGcGgcGGCGUUc -3' miRNA: 3'- gUGUUGGCGUagCAGCaCuuCCGCGA- -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 181 | 0.66 | 0.766807 |
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Target: 5'- cCGCcgGACUGgAUUGUCGgcGAGGCGCg -3' miRNA: 3'- -GUG--UUGGCgUAGCAGCacUUCCGCGa -5' |
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| 24909 | 3' | -54.2 | NC_005284.1 | + | 8135 | 1.07 | 0.001777 |
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Target: 5'- gCACAACCGCAUCGUCGUGAAGGCGCUc -3' miRNA: 3'- -GUGUUGGCGUAGCAGCACUUCCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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