Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24912 | 5' | -53.2 | NC_005284.1 | + | 18737 | 0.66 | 0.804131 |
Target: 5'- cGUCGAcGUcGUAUCAGgGGcUCGCGGu -3' miRNA: 3'- -CGGCU-CGaCAUAGUCgCCuAGCGUUu -5' |
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24912 | 5' | -53.2 | NC_005284.1 | + | 47734 | 0.66 | 0.7841 |
Target: 5'- gGCCGuGCUcggcgGUA-CGGCGGcgCGCGu- -3' miRNA: 3'- -CGGCuCGA-----CAUaGUCGCCuaGCGUuu -5' |
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24912 | 5' | -53.2 | NC_005284.1 | + | 8272 | 0.66 | 0.773828 |
Target: 5'- gGCCGAGCUGUGcgAGCaGAugUCGUc-- -3' miRNA: 3'- -CGGCUCGACAUagUCGcCU--AGCGuuu -5' |
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24912 | 5' | -53.2 | NC_005284.1 | + | 15313 | 0.66 | 0.773828 |
Target: 5'- -gCGGGCg----CAGCGGcAUCGCGAAa -3' miRNA: 3'- cgGCUCGacauaGUCGCC-UAGCGUUU- -5' |
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24912 | 5' | -53.2 | NC_005284.1 | + | 11609 | 0.67 | 0.752831 |
Target: 5'- gGCCGAGC---GUCAGCGuGAcgugcagCGCGAAc -3' miRNA: 3'- -CGGCUCGacaUAGUCGC-CUa------GCGUUU- -5' |
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24912 | 5' | -53.2 | NC_005284.1 | + | 8299 | 0.69 | 0.619369 |
Target: 5'- cGCCGAGCUGaGUCucUGGAUCGgGu- -3' miRNA: 3'- -CGGCUCGACaUAGucGCCUAGCgUuu -5' |
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24912 | 5' | -53.2 | NC_005284.1 | + | 49587 | 0.71 | 0.466167 |
Target: 5'- cGUCGGGCUG-AUCAGCGGucuUCgGCGu- -3' miRNA: 3'- -CGGCUCGACaUAGUCGCCu--AG-CGUuu -5' |
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24912 | 5' | -53.2 | NC_005284.1 | + | 8927 | 1.08 | 0.001548 |
Target: 5'- aGCCGAGCUGUAUCAGCGGAUCGCAAAc -3' miRNA: 3'- -CGGCUCGACAUAGUCGCCUAGCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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