miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
24915 3' -56.1 NC_005284.1 + 3766 0.66 0.658162
Target:  5'- cCGAUCGGaUCauggGCCGGCCUcaaggaggacgacaaGGGUCu -3'
miRNA:   3'- -GCUAGCUaAGgag-CGGCCGGA---------------CCUAG- -5'
24915 3' -56.1 NC_005284.1 + 164 0.66 0.653806
Target:  5'- gCGAUU--UUCCgugcCGUCGGCUUGGGUUu -3'
miRNA:   3'- -GCUAGcuAAGGa---GCGGCCGGACCUAG- -5'
24915 3' -56.1 NC_005284.1 + 19038 0.69 0.473055
Target:  5'- aGAUCGuugCgUCGCCGGCCaucggcgGGGUg -3'
miRNA:   3'- gCUAGCuaaGgAGCGGCCGGa------CCUAg -5'
24915 3' -56.1 NC_005284.1 + 30846 0.71 0.396692
Target:  5'- gCGAUCGcAUggCUCGCCuacccGGCCgcUGGAUCa -3'
miRNA:   3'- -GCUAGC-UAagGAGCGG-----CCGG--ACCUAG- -5'
24915 3' -56.1 NC_005284.1 + 10535 1.1 0.00074
Target:  5'- uCGAUCGAUUCCUCGCCGGCCUGGAUCg -3'
miRNA:   3'- -GCUAGCUAAGGAGCGGCCGGACCUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.