Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24915 | 3' | -56.1 | NC_005284.1 | + | 3766 | 0.66 | 0.658162 |
Target: 5'- cCGAUCGGaUCauggGCCGGCCUcaaggaggacgacaaGGGUCu -3' miRNA: 3'- -GCUAGCUaAGgag-CGGCCGGA---------------CCUAG- -5' |
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24915 | 3' | -56.1 | NC_005284.1 | + | 164 | 0.66 | 0.653806 |
Target: 5'- gCGAUU--UUCCgugcCGUCGGCUUGGGUUu -3' miRNA: 3'- -GCUAGcuAAGGa---GCGGCCGGACCUAG- -5' |
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24915 | 3' | -56.1 | NC_005284.1 | + | 19038 | 0.69 | 0.473055 |
Target: 5'- aGAUCGuugCgUCGCCGGCCaucggcgGGGUg -3' miRNA: 3'- gCUAGCuaaGgAGCGGCCGGa------CCUAg -5' |
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24915 | 3' | -56.1 | NC_005284.1 | + | 30846 | 0.71 | 0.396692 |
Target: 5'- gCGAUCGcAUggCUCGCCuacccGGCCgcUGGAUCa -3' miRNA: 3'- -GCUAGC-UAagGAGCGG-----CCGG--ACCUAG- -5' |
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24915 | 3' | -56.1 | NC_005284.1 | + | 10535 | 1.1 | 0.00074 |
Target: 5'- uCGAUCGAUUCCUCGCCGGCCUGGAUCg -3' miRNA: 3'- -GCUAGCUAAGGAGCGGCCGGACCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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