Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
24923 | 3' | -58.8 | NC_005284.1 | + | 14244 | 0.66 | 0.525387 |
Target: 5'- uGUGCuguuGGCCGGguuGCUUGAgacggaugCGGCGu -3' miRNA: 3'- gCACGu---CCGGCUac-CGAGCUa-------GCCGU- -5' |
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24923 | 3' | -58.8 | NC_005284.1 | + | 26015 | 0.66 | 0.525387 |
Target: 5'- uCGcGCAaauuauGCCGG-GGCUCGAugUCGGCGa -3' miRNA: 3'- -GCaCGUc-----CGGCUaCCGAGCU--AGCCGU- -5' |
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24923 | 3' | -58.8 | NC_005284.1 | + | 38340 | 0.66 | 0.514031 |
Target: 5'- uGUGcCGGGCgCGcUGGCggcaccaggacaaUCGAUCGaGCAa -3' miRNA: 3'- gCAC-GUCCG-GCuACCG-------------AGCUAGC-CGU- -5' |
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24923 | 3' | -58.8 | NC_005284.1 | + | 30371 | 0.66 | 0.52228 |
Target: 5'- --cGCgAGGgCGAUGcGCUCGGcccgcgugugcuggUCGGCAu -3' miRNA: 3'- gcaCG-UCCgGCUAC-CGAGCU--------------AGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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