miRNA: 3'- -GCa-CGUCCGGCuaCCGAGCuAGCCGu -5'
miRNA: 3'- -GC-ACGUCCGGCU-ACCGaGCuAGCCGU- -5'
miRNA: 3'- gCACGuCCGGCUAC-CGAGCUaGCCGU- -5'
miRNA: 3'- -GCACGUCCGGCUACCGAGCUAGCCGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24923 | 3' | -58.8 | NC_005284.1 | + | 45641 | 0.75 | 0.145841 |
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Target: 5'- gCGggGCAGGCUGgcGGCUCG-UCGGUg -3' miRNA: 3'- -GCa-CGUCCGGCuaCCGAGCuAGCCGu -5' |
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| 24923 | 3' | -58.8 | NC_005284.1 | + | 11856 | 0.77 | 0.104967 |
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Target: 5'- gCGuUGCAGGCCGAcUGGCgCG-UCGGCGu -3' miRNA: 3'- -GC-ACGUCCGGCU-ACCGaGCuAGCCGU- -5' |
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| 24923 | 3' | -58.8 | NC_005284.1 | + | 16880 | 0.8 | 0.063337 |
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Target: 5'- gGUGCuGGCCGGUGcGUUCGA-CGGCAa -3' miRNA: 3'- gCACGuCCGGCUAC-CGAGCUaGCCGU- -5' |
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| 24923 | 3' | -58.8 | NC_005284.1 | + | 15355 | 1.08 | 0.000519 |
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Target: 5'- gCGUGCAGGCCGAUGGCUCGAUCGGCAu -3' miRNA: 3'- -GCACGUCCGGCUACCGAGCUAGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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