Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24923 | 3' | -58.8 | NC_005284.1 | + | 45641 | 0.75 | 0.145841 |
Target: 5'- gCGggGCAGGCUGgcGGCUCG-UCGGUg -3' miRNA: 3'- -GCa-CGUCCGGCuaCCGAGCuAGCCGu -5' |
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24923 | 3' | -58.8 | NC_005284.1 | + | 47217 | 0.67 | 0.455026 |
Target: 5'- gCGUGCAGGgCGu--GCUCGAgcCGGUg -3' miRNA: 3'- -GCACGUCCgGCuacCGAGCUa-GCCGu -5' |
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24923 | 3' | -58.8 | NC_005284.1 | + | 47461 | 0.67 | 0.474636 |
Target: 5'- ---cCGGGCCGAgaagaUGGCUCGcaugcucgacGUCGGCc -3' miRNA: 3'- gcacGUCCGGCU-----ACCGAGC----------UAGCCGu -5' |
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24923 | 3' | -58.8 | NC_005284.1 | + | 47722 | 0.67 | 0.484599 |
Target: 5'- gCGUGCgcagaAGGCCGu--GCUCGG-CGGUAc -3' miRNA: 3'- -GCACG-----UCCGGCuacCGAGCUaGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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