Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24926 | 3' | -58.1 | NC_005284.1 | + | 37165 | 0.66 | 0.510809 |
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Target: 5'- --cGCCCugaaucucgccGGCGCGUcgaugGCGGUCGAGg- -3' miRNA: 3'- uaaCGGGu----------CCGCGCG-----CGUCAGUUCag -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 48334 | 0.66 | 0.48998 |
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Target: 5'- -gUGCCCGcacGGCaaCGCGCcGUCAGcGUCa -3' miRNA: 3'- uaACGGGU---CCGc-GCGCGuCAGUU-CAG- -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 42976 | 0.66 | 0.479715 |
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Target: 5'- uUUGCCgCGGGgGCGUuGCAGUCcguGAGcCu -3' miRNA: 3'- uAACGG-GUCCgCGCG-CGUCAG---UUCaG- -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 8967 | 0.67 | 0.459508 |
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Target: 5'- gGUUGCCguGGuCGCGCGCGaugcgcagCAcGUCg -3' miRNA: 3'- -UAACGGguCC-GCGCGCGUca------GUuCAG- -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 48156 | 0.67 | 0.420511 |
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Target: 5'- --aGCCCAuuccGGCGCGCGCuGgcgAAGUg -3' miRNA: 3'- uaaCGGGU----CCGCGCGCGuCag-UUCAg -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 49133 | 0.68 | 0.39261 |
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Target: 5'- -aUGCgUuuuaAGGCGCGCGUcGUCAAGg- -3' miRNA: 3'- uaACGgG----UCCGCGCGCGuCAGUUCag -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 31517 | 0.68 | 0.365941 |
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Target: 5'- --gGCCC-GGCGCGCcGCAGcggcgCAcGUCg -3' miRNA: 3'- uaaCGGGuCCGCGCG-CGUCa----GUuCAG- -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 9746 | 0.72 | 0.215288 |
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Target: 5'- cUUGCCgAaGCGCGgGCGGUCGAGg- -3' miRNA: 3'- uAACGGgUcCGCGCgCGUCAGUUCag -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 6543 | 0.73 | 0.173573 |
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Target: 5'- --cGCUCAGGCGCGCGaCAGUgGcuGGUg -3' miRNA: 3'- uaaCGGGUCCGCGCGC-GUCAgU--UCAg -5' |
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| 24926 | 3' | -58.1 | NC_005284.1 | + | 17991 | 1.08 | 0.000483 |
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Target: 5'- uAUUGCCCAGGCGCGCGCAGUCAAGUCg -3' miRNA: 3'- -UAACGGGUCCGCGCGCGUCAGUUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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