Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24932 | 3' | -57.8 | NC_005284.1 | + | 18977 | 0.66 | 0.606998 |
Target: 5'- aACGAucuACGcGGGCGacgaaaggcucaauGCUGGCGUGUGg -3' miRNA: 3'- -UGCUcuuUGU-CCCGC--------------UGGCCGCACGC- -5' |
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24932 | 3' | -57.8 | NC_005284.1 | + | 10070 | 0.66 | 0.599473 |
Target: 5'- aGCGAGcgGAACGcGcGGCGACCGcgaccGCG-GCGg -3' miRNA: 3'- -UGCUC--UUUGU-C-CCGCUGGC-----CGCaCGC- -5' |
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24932 | 3' | -57.8 | NC_005284.1 | + | 2622 | 0.66 | 0.599473 |
Target: 5'- cGCGAGaAAGCuccGGuCGGCgGGUGUGCu -3' miRNA: 3'- -UGCUC-UUUGuc-CC-GCUGgCCGCACGc -5' |
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24932 | 3' | -57.8 | NC_005284.1 | + | 8744 | 0.66 | 0.593034 |
Target: 5'- cCGcGAAuacgcacaagccgccGCAGcGGCGuuCGGCGUGCa -3' miRNA: 3'- uGCuCUU---------------UGUC-CCGCugGCCGCACGc -5' |
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24932 | 3' | -57.8 | NC_005284.1 | + | 10660 | 0.66 | 0.578055 |
Target: 5'- cGCGAGGAACuGGuGCGcGCCGcGgGcGCGa -3' miRNA: 3'- -UGCUCUUUGuCC-CGC-UGGC-CgCaCGC- -5' |
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24932 | 3' | -57.8 | NC_005284.1 | + | 47781 | 0.66 | 0.567407 |
Target: 5'- uCGAGGcggcgcucGCGGaGGCGACgGGCGaGCGc -3' miRNA: 3'- uGCUCUu-------UGUC-CCGCUGgCCGCaCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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