Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24932 | 3' | -57.8 | NC_005284.1 | + | 32808 | 0.68 | 0.445387 |
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Target: 5'- cGCGGGuuGCGGGGCGGCguCGGUuacgGCa -3' miRNA: 3'- -UGCUCuuUGUCCCGCUG--GCCGca--CGc -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 33981 | 0.69 | 0.435863 |
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Target: 5'- uGCGGGcugcucGACAGGGgGugCGGCGaggGCc -3' miRNA: 3'- -UGCUCu-----UUGUCCCgCugGCCGCa--CGc -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 34199 | 0.67 | 0.49466 |
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Target: 5'- uACGAc---CAuGuGGCGAUCGGCGUGCa -3' miRNA: 3'- -UGCUcuuuGU-C-CCGCUGGCCGCACGc -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 45630 | 0.72 | 0.259479 |
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Target: 5'- uGCGAGucuucGCGGGGCaGGCUGGCG-GCu -3' miRNA: 3'- -UGCUCuu---UGUCCCG-CUGGCCGCaCGc -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 47781 | 0.66 | 0.567407 |
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Target: 5'- uCGAGGcggcgcucGCGGaGGCGACgGGCGaGCGc -3' miRNA: 3'- uGCUCUu-------UGUC-CCGCUGgCCGCaCGC- -5' |
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| 24932 | 3' | -57.8 | NC_005284.1 | + | 52736 | 0.67 | 0.504816 |
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Target: 5'- ------uACGGGGUGGCCGGUGUGg- -3' miRNA: 3'- ugcucuuUGUCCCGCUGGCCGCACgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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