Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24933 | 3' | -56.4 | NC_005284.1 | + | 54684 | 0.67 | 0.585762 |
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Target: 5'- aGAcGGCgcgcgaGCGGUUGCGgUGACGugGu -3' miRNA: 3'- aCUaCCGa-----CGUCGACGCaGCUGCugC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 43138 | 0.67 | 0.595571 |
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Target: 5'- aGAUGGaUUGCAGgggacguCUGCGgugacaUUGACGACGc -3' miRNA: 3'- aCUACC-GACGUC-------GACGC------AGCUGCUGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 33461 | 0.67 | 0.596662 |
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Target: 5'- gUGAUGGCgagccGuCGGCaGCGcCGGcCGACGa -3' miRNA: 3'- -ACUACCGa----C-GUCGaCGCaGCU-GCUGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 31808 | 0.66 | 0.640472 |
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Target: 5'- cGcUGGCUGCGGgaGCGagcaugguaugUCGAUG-CGg -3' miRNA: 3'- aCuACCGACGUCgaCGC-----------AGCUGCuGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 29618 | 0.66 | 0.640472 |
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Target: 5'- aUGAcGGCuccguuUGCGGCUGUGaCGACGuuGa -3' miRNA: 3'- -ACUaCCG------ACGUCGACGCaGCUGCugC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 26752 | 0.66 | 0.651429 |
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Target: 5'- ----aGCUcgAGCUGCGUCGACGAg- -3' miRNA: 3'- acuacCGAcgUCGACGCAGCUGCUgc -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 53578 | 0.66 | 0.65909 |
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Target: 5'- cUGggGGCgUGCGGCUGUGgCGgucaucgaguucauGCGACu -3' miRNA: 3'- -ACuaCCG-ACGUCGACGCaGC--------------UGCUGc -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 21869 | 0.66 | 0.662369 |
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Target: 5'- ---cGGCUGCcguGCaGUGUCGgacaGCGACGc -3' miRNA: 3'- acuaCCGACGu--CGaCGCAGC----UGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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