Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24933 | 3' | -56.4 | NC_005284.1 | + | 17765 | 0.69 | 0.47003 |
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Target: 5'- cGcUGGCUGCuGCUGaCGUCG-CGguACGa -3' miRNA: 3'- aCuACCGACGuCGAC-GCAGCuGC--UGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 14585 | 0.71 | 0.367093 |
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Target: 5'- cGAUGGCUGCuGC-GCGUCgGAUGuCu -3' miRNA: 3'- aCUACCGACGuCGaCGCAG-CUGCuGc -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 37109 | 0.7 | 0.421327 |
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Target: 5'- aGgcGGCacuacaGCAGC-GCGUCGAgGACGa -3' miRNA: 3'- aCuaCCGa-----CGUCGaCGCAGCUgCUGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 23394 | 0.68 | 0.500591 |
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Target: 5'- uUGAUGcaacGCAGUUcCGUCGACGACGc -3' miRNA: 3'- -ACUACcga-CGUCGAcGCAGCUGCUGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 33461 | 0.67 | 0.596662 |
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Target: 5'- gUGAUGGCgagccGuCGGCaGCGcCGGcCGACGa -3' miRNA: 3'- -ACUACCGa----C-GUCGaCGCaGCU-GCUGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 31808 | 0.66 | 0.640472 |
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Target: 5'- cGcUGGCUGCGGgaGCGagcaugguaugUCGAUG-CGg -3' miRNA: 3'- aCuACCGACGUCgaCGC-----------AGCUGCuGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 21869 | 0.66 | 0.662369 |
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Target: 5'- ---cGGCUGCcguGCaGUGUCGgacaGCGACGc -3' miRNA: 3'- acuaCCGACGu--CGaCGCAGC----UGCUGC- -5' |
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| 24933 | 3' | -56.4 | NC_005284.1 | + | 18336 | 0.73 | 0.287891 |
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Target: 5'- cGAcGGCgGCGGCUGCacCGAUGGCGg -3' miRNA: 3'- aCUaCCGaCGUCGACGcaGCUGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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