Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24934 | 3' | -54.7 | NC_005284.1 | + | 19141 | 0.66 | 0.761672 |
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Target: 5'- uGGGCcgcGACUGUUUCGacuuuCUUCGcGACCg -3' miRNA: 3'- -CCCGcuaCUGACAAGGU-----GAGGC-CUGG- -5' |
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| 24934 | 3' | -54.7 | NC_005284.1 | + | 33464 | 0.66 | 0.761672 |
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Target: 5'- cGGGUGAUGGCgagccgUCgGCagcgCCGG-CCg -3' miRNA: 3'- -CCCGCUACUGaca---AGgUGa---GGCCuGG- -5' |
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| 24934 | 3' | -54.7 | NC_005284.1 | + | 7019 | 0.67 | 0.741164 |
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Target: 5'- cGGGC--UGACUGgcggCCGUUCCGGuuCCu -3' miRNA: 3'- -CCCGcuACUGACaa--GGUGAGGCCu-GG- -5' |
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| 24934 | 3' | -54.7 | NC_005284.1 | + | 5273 | 0.68 | 0.666568 |
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Target: 5'- aGGCGAUGACUGcgaacgaaUUCCucgUCGGcGCCu -3' miRNA: 3'- cCCGCUACUGAC--------AAGGugaGGCC-UGG- -5' |
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| 24934 | 3' | -54.7 | NC_005284.1 | + | 14588 | 0.7 | 0.515814 |
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Target: 5'- cGGCGAUGGCUGcUgCGCgUCGGAUg -3' miRNA: 3'- cCCGCUACUGACaAgGUGaGGCCUGg -5' |
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| 24934 | 3' | -54.7 | NC_005284.1 | + | 54574 | 0.8 | 0.151911 |
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Target: 5'- aGGGCGcGUGACUGUcgCCACUgCGGuugACCa -3' miRNA: 3'- -CCCGC-UACUGACAa-GGUGAgGCC---UGG- -5' |
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| 24934 | 3' | -54.7 | NC_005284.1 | + | 23744 | 1.15 | 0.000556 |
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Target: 5'- uGGGCGAUGACUGUUCCACUCCGGACCa -3' miRNA: 3'- -CCCGCUACUGACAAGGUGAGGCCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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