Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24934 | 5' | -51.2 | NC_005284.1 | + | 21562 | 0.66 | 0.906466 |
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Target: 5'- --cGACGAGCA----GGCCGCG-CGg -3' miRNA: 3'- guuCUGCUCGUcuaaUCGGCGUaGCa -5' |
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| 24934 | 5' | -51.2 | NC_005284.1 | + | 13789 | 0.66 | 0.884513 |
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Target: 5'- uGGGuuGGGCGGGUUgAGCUGCcUCGg -3' miRNA: 3'- gUUCugCUCGUCUAA-UCGGCGuAGCa -5' |
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| 24934 | 5' | -51.2 | NC_005284.1 | + | 3567 | 0.66 | 0.868477 |
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Target: 5'- uCAAGGCGuccugcGCAGccgUAGCCGUcgUGUg -3' miRNA: 3'- -GUUCUGCu-----CGUCua-AUCGGCGuaGCA- -5' |
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| 24934 | 5' | -51.2 | NC_005284.1 | + | 10962 | 0.67 | 0.84246 |
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Target: 5'- -cGGACGAGCGGAcgcaucaAGUCGC-UCGa -3' miRNA: 3'- guUCUGCUCGUCUaa-----UCGGCGuAGCa -5' |
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| 24934 | 5' | -51.2 | NC_005284.1 | + | 38911 | 0.69 | 0.741951 |
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Target: 5'- gCAGGGCGGGCGGGUUGGUUagGCGgcuucccguUCGg -3' miRNA: 3'- -GUUCUGCUCGUCUAAUCGG--CGU---------AGCa -5' |
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| 24934 | 5' | -51.2 | NC_005284.1 | + | 16299 | 0.71 | 0.606665 |
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Target: 5'- gCAGGGCGAGCcGGU--GCCGC-UCGUc -3' miRNA: 3'- -GUUCUGCUCGuCUAauCGGCGuAGCA- -5' |
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| 24934 | 5' | -51.2 | NC_005284.1 | + | 7447 | 0.73 | 0.527983 |
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Target: 5'- --cGACGAacGcCAGAUcGGCCGCGUCGa -3' miRNA: 3'- guuCUGCU--C-GUCUAaUCGGCGUAGCa -5' |
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| 24934 | 5' | -51.2 | NC_005284.1 | + | 23216 | 0.76 | 0.348969 |
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Target: 5'- --cGACGGGCGGuggAGCCGUGUCGa -3' miRNA: 3'- guuCUGCUCGUCuaaUCGGCGUAGCa -5' |
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| 24934 | 5' | -51.2 | NC_005284.1 | + | 23778 | 1.07 | 0.003265 |
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Target: 5'- gCAAGACGAGCAGAUUAGCCGCAUCGUc -3' miRNA: 3'- -GUUCUGCUCGUCUAAUCGGCGUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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