Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24935 | 3' | -55.4 | NC_005284.1 | + | 9451 | 0.66 | 0.671598 |
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Target: 5'- cGGGCGGAuuGCCGUGUacuuUGGgcaauccgcagucgcGGCGACGCa -3' miRNA: 3'- -UCUGUUU--CGGCGCAc---ACC---------------CCGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 54366 | 0.66 | 0.689095 |
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Target: 5'- cGAUGAAGCCGUGcaagcaccgGGGGUGcgguGCGCu -3' miRNA: 3'- uCUGUUUCGGCGCaca------CCCCGU----UGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 15722 | 0.66 | 0.689095 |
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Target: 5'- cGGGCGucugucgacGCgCGCgGUGUGGGGCGgaAUGUg -3' miRNA: 3'- -UCUGUuu-------CG-GCG-CACACCCCGU--UGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 33114 | 0.66 | 0.699962 |
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Target: 5'- cGGGCGAugcuGCCucuGCGUGaacggguugaGGGGCGGCuGCa -3' miRNA: 3'- -UCUGUUu---CGG---CGCACa---------CCCCGUUG-CG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 10083 | 0.66 | 0.699962 |
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Target: 5'- -cGCAAGGCCGCGguccGUcucGGccaGCGACGCc -3' miRNA: 3'- ucUGUUUCGGCGCa---CA---CCc--CGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 18992 | 0.66 | 0.710763 |
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Target: 5'- cGACGAaaGGCUcaaugcugGCGUGUGGucgcugcaGGCGGCGa -3' miRNA: 3'- uCUGUU--UCGG--------CGCACACC--------CCGUUGCg -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 11013 | 0.66 | 0.710763 |
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Target: 5'- cGACGAGGCCGgccaUGcGGGCcuGCGCa -3' miRNA: 3'- uCUGUUUCGGCgcacAC-CCCGu-UGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 3390 | 0.66 | 0.710763 |
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Target: 5'- cGGGCGAAggaaaaccuGCCGCcgaUG-GGuGGCAAUGCa -3' miRNA: 3'- -UCUGUUU---------CGGCGc--ACaCC-CCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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