Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24935 | 3' | -55.4 | NC_005284.1 | + | 22243 | 0.69 | 0.50418 |
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Target: 5'- cGuCGAGGUguuCGCGgGcGGGGCGGCGCu -3' miRNA: 3'- uCuGUUUCG---GCGCaCaCCCCGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 15293 | 0.69 | 0.493792 |
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Target: 5'- cGACGAGGaCCGCacgauGUGcGGGcGCAGCGg -3' miRNA: 3'- uCUGUUUC-GGCG-----CACaCCC-CGUUGCg -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 18830 | 0.7 | 0.433716 |
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Target: 5'- cGGCGAaggGGCCggGCGUGcaGGGGCAGCcgaGCa -3' miRNA: 3'- uCUGUU---UCGG--CGCACa-CCCCGUUG---CG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 15242 | 0.71 | 0.414649 |
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Target: 5'- cGGCAcGGCCGCaagGUuGcGGCAGCGCa -3' miRNA: 3'- uCUGUuUCGGCGca-CAcC-CCGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 40465 | 0.71 | 0.387037 |
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Target: 5'- aGGGCAAGuucGaCCGCGUGUGGcGaaGACGCg -3' miRNA: 3'- -UCUGUUU---C-GGCGCACACC-CcgUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 6814 | 0.71 | 0.387037 |
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Target: 5'- cGGCGAGGCCGag---GGGGCGAuuCGCa -3' miRNA: 3'- uCUGUUUCGGCgcacaCCCCGUU--GCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 45654 | 0.74 | 0.275006 |
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Target: 5'- cAGGCGucgauAGCCGaGUGcGcGGGCGACGCg -3' miRNA: 3'- -UCUGUu----UCGGCgCACaC-CCCGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 24320 | 0.99 | 0.004811 |
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Target: 5'- gAGACAAGaCCGCGUGUGGGGCAACGCu -3' miRNA: 3'- -UCUGUUUcGGCGCACACCCCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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