Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24935 | 3' | -55.4 | NC_005284.1 | + | 24825 | 0.68 | 0.590096 |
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Target: 5'- cGACcGGGUCGgGUGUaGcGGGCcgaaGACGCa -3' miRNA: 3'- uCUGuUUCGGCgCACA-C-CCCG----UUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 33114 | 0.66 | 0.699962 |
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Target: 5'- cGGGCGAugcuGCCucuGCGUGaacggguugaGGGGCGGCuGCa -3' miRNA: 3'- -UCUGUUu---CGG---CGCACa---------CCCCGUUG-CG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 35803 | 0.67 | 0.656207 |
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Target: 5'- cGGCGcuugAAGCCGUGcaUGGGGCcgacaaacAGCGCc -3' miRNA: 3'- uCUGU----UUCGGCGCacACCCCG--------UUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 40465 | 0.71 | 0.387037 |
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Target: 5'- aGGGCAAGuucGaCCGCGUGUGGcGaaGACGCg -3' miRNA: 3'- -UCUGUUU---C-GGCGCACACC-CcgUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 45654 | 0.74 | 0.275006 |
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Target: 5'- cAGGCGucgauAGCCGaGUGcGcGGGCGACGCg -3' miRNA: 3'- -UCUGUu----UCGGCgCACaC-CCCGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 50828 | 0.67 | 0.645186 |
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Target: 5'- cGAU--GGCCGUuggGUGGGGCcgGGCGUu -3' miRNA: 3'- uCUGuuUCGGCGca-CACCCCG--UUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 54366 | 0.66 | 0.689095 |
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Target: 5'- cGAUGAAGCCGUGcaagcaccgGGGGUGcgguGCGCu -3' miRNA: 3'- uCUGUUUCGGCGCaca------CCCCGU----UGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 54493 | 0.68 | 0.568245 |
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Target: 5'- aGGAUAcGGaaCCGCGUG-GGacaagaucaGGCAGCGCa -3' miRNA: 3'- -UCUGUuUC--GGCGCACaCC---------CCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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