Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24935 | 3' | -55.4 | NC_005284.1 | + | 54493 | 0.68 | 0.568245 |
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Target: 5'- aGGAUAcGGaaCCGCGUG-GGacaagaucaGGCAGCGCa -3' miRNA: 3'- -UCUGUuUC--GGCGCACaCC---------CCGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 10235 | 0.67 | 0.612089 |
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Target: 5'- uGGGCAcGGUgGCGcuUGGGGCugcCGCg -3' miRNA: 3'- -UCUGUuUCGgCGCacACCCCGuu-GCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 7940 | 0.67 | 0.612089 |
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Target: 5'- gGGACAucGCCGgGguaacgGaGGGCGACGa -3' miRNA: 3'- -UCUGUuuCGGCgCaca---C-CCCGUUGCg -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 15242 | 0.71 | 0.414649 |
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Target: 5'- cGGCAcGGCCGCaagGUuGcGGCAGCGCa -3' miRNA: 3'- uCUGUuUCGGCGca-CAcC-CCGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 24825 | 0.68 | 0.590096 |
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Target: 5'- cGACcGGGUCGgGUGUaGcGGGCcgaaGACGCa -3' miRNA: 3'- uCUGuUUCGGCgCACA-C-CCCG----UUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 10083 | 0.66 | 0.699962 |
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Target: 5'- -cGCAAGGCCGCGguccGUcucGGccaGCGACGCc -3' miRNA: 3'- ucUGUUUCGGCGCa---CA---CCc--CGUUGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 11013 | 0.66 | 0.710763 |
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Target: 5'- cGACGAGGCCGgccaUGcGGGCcuGCGCa -3' miRNA: 3'- uCUGUUUCGGCgcacAC-CCCGu-UGCG- -5' |
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| 24935 | 3' | -55.4 | NC_005284.1 | + | 45654 | 0.74 | 0.275006 |
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Target: 5'- cAGGCGucgauAGCCGaGUGcGcGGGCGACGCg -3' miRNA: 3'- -UCUGUu----UCGGCgCACaC-CCCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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