Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24938 | 3' | -57.8 | NC_005284.1 | + | 27743 | 0.66 | 0.551091 |
Target: 5'- aGACCGGccgaaacgucUGGcaucgAAUCGGccugacugauuGGCGGCCGUa -3' miRNA: 3'- gUUGGCC----------ACC-----UUAGCC-----------UCGCCGGCAg -5' |
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24938 | 3' | -57.8 | NC_005284.1 | + | 53878 | 0.67 | 0.499197 |
Target: 5'- aCAGCgGccGGGAU-GG-GCGGCCGUCg -3' miRNA: 3'- -GUUGgCcaCCUUAgCCuCGCCGGCAG- -5' |
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24938 | 3' | -57.8 | NC_005284.1 | + | 32790 | 0.67 | 0.496149 |
Target: 5'- -uGCCGGUgcgucggccugcgcGGGuugCGGGGCGG-CGUCg -3' miRNA: 3'- guUGGCCA--------------CCUua-GCCUCGCCgGCAG- -5' |
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24938 | 3' | -57.8 | NC_005284.1 | + | 12141 | 0.69 | 0.402821 |
Target: 5'- -cGCuCGGUGGug-CGGcaagcGCGGCUGUCg -3' miRNA: 3'- guUG-GCCACCuuaGCCu----CGCCGGCAG- -5' |
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24938 | 3' | -57.8 | NC_005284.1 | + | 25511 | 1.09 | 0.00055 |
Target: 5'- gCAACCGGUGGAAUCGGAGCGGCCGUCg -3' miRNA: 3'- -GUUGGCCACCUUAGCCUCGCCGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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