Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24939 | 3' | -57.1 | NC_005284.1 | + | 45690 | 0.69 | 0.407552 |
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Target: 5'- cACGCCUUcaCGaCCGCGCgUCGACa--- -3' miRNA: 3'- -UGCGGAGaaGC-GGCGCG-AGCUGaagu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 49092 | 0.7 | 0.354805 |
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Target: 5'- cCGCUcg-UCGCCGUGCUgucUGACUUCAc -3' miRNA: 3'- uGCGGagaAGCGGCGCGA---GCUGAAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 10979 | 0.71 | 0.338325 |
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Target: 5'- cGCGCC-CUUCGuCCGCGCUuCGuACUg-- -3' miRNA: 3'- -UGCGGaGAAGC-GGCGCGA-GC-UGAagu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 21607 | 0.72 | 0.278049 |
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Target: 5'- aGCGUugggcaagauCUCgcgCGCCGCGCUCGACg--- -3' miRNA: 3'- -UGCG----------GAGaa-GCGGCGCGAGCUGaagu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 53497 | 0.73 | 0.238692 |
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Target: 5'- cCGCCggUUCGCCGCGCggGGCUUUu -3' miRNA: 3'- uGCGGagAAGCGGCGCGagCUGAAGu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 10196 | 0.74 | 0.22082 |
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Target: 5'- uGCGCCg--UCGCCGCGCUuucggucgcCGuCUUCAg -3' miRNA: 3'- -UGCGGagaAGCGGCGCGA---------GCuGAAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 54747 | 0.74 | 0.198778 |
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Target: 5'- cGCGCCgUCUUCGCCGCGU--GACagUCAc -3' miRNA: 3'- -UGCGG-AGAAGCGGCGCGagCUGa-AGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 14759 | 0.75 | 0.188492 |
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Target: 5'- cACG-CUC-UCGUCGcCGCUCGACUUCGg -3' miRNA: 3'- -UGCgGAGaAGCGGC-GCGAGCUGAAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 26669 | 1.08 | 0.000748 |
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Target: 5'- aACGCCUCUUCGCCGCGCUCGACUUCAa -3' miRNA: 3'- -UGCGGAGAAGCGGCGCGAGCUGAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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