Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24939 | 3' | -57.1 | NC_005284.1 | + | 15219 | 0.67 | 0.526265 |
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Target: 5'- aGCGCacguauaUC-UCGCCGCuCUCGGuCUUCAc -3' miRNA: 3'- -UGCGg------AGaAGCGGCGcGAGCU-GAAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 15565 | 0.67 | 0.526265 |
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Target: 5'- aACGCga---CGCCGCGCUCGGCg--- -3' miRNA: 3'- -UGCGgagaaGCGGCGCGAGCUGaagu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 11868 | 0.67 | 0.536794 |
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Target: 5'- cCGCCUCggcCGUguugaUGCGCUCGAUcUCGg -3' miRNA: 3'- uGCGGAGaa-GCG-----GCGCGAGCUGaAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 47736 | 0.67 | 0.547395 |
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Target: 5'- aACGCCUgaacCUUCGCgCGuUGCUCGucgggcgacaGCUUCGc -3' miRNA: 3'- -UGCGGA----GAAGCG-GC-GCGAGC----------UGAAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 11344 | 0.66 | 0.579561 |
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Target: 5'- cCGCCcccCUUCGCCGCGCgagCcGCUg-- -3' miRNA: 3'- uGCGGa--GAAGCGGCGCGa--GcUGAagu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 38166 | 0.66 | 0.612109 |
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Target: 5'- uCGCgUCUUCGuCCGCGCgacgagccacaUCGGCa--- -3' miRNA: 3'- uGCGgAGAAGC-GGCGCG-----------AGCUGaagu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 6163 | 0.66 | 0.623004 |
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Target: 5'- -aGCaagCUCUUCGCCcCGCUCGGaagUUCGg -3' miRNA: 3'- ugCG---GAGAAGCGGcGCGAGCUg--AAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 37167 | 0.66 | 0.633907 |
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Target: 5'- gACGCC-CUgaaucUCGCCgGCGCgUCGAUggCGg -3' miRNA: 3'- -UGCGGaGA-----AGCGG-CGCG-AGCUGaaGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 54747 | 0.74 | 0.198778 |
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Target: 5'- cGCGCCgUCUUCGCCGCGU--GACagUCAc -3' miRNA: 3'- -UGCGG-AGAAGCGGCGCGagCUGa-AGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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