Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24939 | 3' | -57.1 | NC_005284.1 | + | 45690 | 0.69 | 0.407552 |
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Target: 5'- cACGCCUUcaCGaCCGCGCgUCGACa--- -3' miRNA: 3'- -UGCGGAGaaGC-GGCGCG-AGCUGaagu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 35449 | 0.69 | 0.435703 |
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Target: 5'- -aGCCUUgUUCGUCGCGCUgGAUcUCGc -3' miRNA: 3'- ugCGGAG-AAGCGGCGCGAgCUGaAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 44938 | 0.69 | 0.435703 |
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Target: 5'- cGCGCCUCgguguaUCGCC-CGC-CGuGCUUCGg -3' miRNA: 3'- -UGCGGAGa-----AGCGGcGCGaGC-UGAAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 9905 | 0.68 | 0.464944 |
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Target: 5'- cGCGCCgaagCgUCGCuCGCGCUCGcGCagCAg -3' miRNA: 3'- -UGCGGa---GaAGCG-GCGCGAGC-UGaaGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 41945 | 0.67 | 0.536794 |
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Target: 5'- uGCGCCaCUgaugauugUCGUCGCGCgUCuACUUCAg -3' miRNA: 3'- -UGCGGaGA--------AGCGGCGCG-AGcUGAAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 15490 | 0.67 | 0.536794 |
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Target: 5'- uCGCCgagcgCggCGUCGCGUUaccCGACUUCGa -3' miRNA: 3'- uGCGGa----GaaGCGGCGCGA---GCUGAAGU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 8230 | 0.66 | 0.601231 |
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Target: 5'- cCGCUUCcugaugUCGCuCGCGCUgCGGCUcggCAa -3' miRNA: 3'- uGCGGAGa-----AGCG-GCGCGA-GCUGAa--GU- -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 30129 | 0.66 | 0.612109 |
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Target: 5'- cACGUCgaCUgcaCGCCGCGCgCGACUg-- -3' miRNA: 3'- -UGCGGa-GAa--GCGGCGCGaGCUGAagu -5' |
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| 24939 | 3' | -57.1 | NC_005284.1 | + | 26669 | 1.08 | 0.000748 |
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Target: 5'- aACGCCUCUUCGCCGCGCUCGACUUCAa -3' miRNA: 3'- -UGCGGAGAAGCGGCGCGAGCUGAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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