Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 24943 | 3' | -60.1 | NC_005284.1 | + | 14237 | 0.66 | 0.468936 |
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Target: 5'- cCGUCGguguGCUGUugGCCGgGUugcuugagacggauGCGGCg -3' miRNA: 3'- uGCAGC----UGACGugCGGCgCG--------------CGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 41031 | 0.66 | 0.471856 |
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Target: 5'- gACGUucaCGcauACUGCGCGCCGacguuucuCGCGgGGCg -3' miRNA: 3'- -UGCA---GC---UGACGUGCGGC--------GCGCgCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 10385 | 0.66 | 0.440271 |
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Target: 5'- -aG-CGGCUGCgcacgaagcaagagGCGUCGCGgGCGGCc -3' miRNA: 3'- ugCaGCUGACG--------------UGCGGCGCgCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 51348 | 0.66 | 0.443093 |
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Target: 5'- cCGuUCGAa-GCACGUucuuCGCGCGUGGCUc -3' miRNA: 3'- uGC-AGCUgaCGUGCG----GCGCGCGCUGA- -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 15937 | 0.66 | 0.491546 |
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Target: 5'- gGCGUUc-CUG-ACGUCgGCGCGCGACg -3' miRNA: 3'- -UGCAGcuGACgUGCGG-CGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 54767 | 0.66 | 0.490552 |
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Target: 5'- cACGUCaccgcaaccGCUcGCGCGCCGUcuucgccGCGUGACa -3' miRNA: 3'- -UGCAGc--------UGA-CGUGCGGCG-------CGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 31418 | 0.66 | 0.452572 |
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Target: 5'- aGCG-CGuguuCUGCGCGUCGCuCGcCGACg -3' miRNA: 3'- -UGCaGCu---GACGUGCGGCGcGC-GCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 49712 | 0.66 | 0.452572 |
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Target: 5'- cCGUCGGaacGC-CGCUGaCGCGCGAUc -3' miRNA: 3'- uGCAGCUga-CGuGCGGC-GCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 46484 | 0.66 | 0.490552 |
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Target: 5'- gGCGUUaGCUGCucgugcauggccgACGCCGCaCGgGACg -3' miRNA: 3'- -UGCAGcUGACG-------------UGCGGCGcGCgCUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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