Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24943 | 3' | -60.1 | NC_005284.1 | + | 48418 | 0.68 | 0.354121 |
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Target: 5'- -aGUUGGCUGCGaagacgcgccaauCGCCGCGUGCa--- -3' miRNA: 3'- ugCAGCUGACGU-------------GCGGCGCGCGcuga -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 48830 | 0.69 | 0.331013 |
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Target: 5'- uCG-CGGC-GCACGCCGCGCGgguUGAUg -3' miRNA: 3'- uGCaGCUGaCGUGCGGCGCGC---GCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 49329 | 0.72 | 0.201877 |
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Target: 5'- -gGUUGGCacgGCACG-CGUGCGCGACg -3' miRNA: 3'- ugCAGCUGa--CGUGCgGCGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 49472 | 0.7 | 0.27312 |
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Target: 5'- --uUCGGCUGCuuGCC-CGUGCGGCg -3' miRNA: 3'- ugcAGCUGACGugCGGcGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 49712 | 0.66 | 0.452572 |
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Target: 5'- cCGUCGGaacGC-CGCUGaCGCGCGAUc -3' miRNA: 3'- uGCAGCUga-CGuGCGGC-GCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 51348 | 0.66 | 0.443093 |
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Target: 5'- cCGuUCGAa-GCACGUucuuCGCGCGUGGCUc -3' miRNA: 3'- uGC-AGCUgaCGUGCG----GCGCGCGCUGA- -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 51644 | 0.73 | 0.159568 |
|
Target: 5'- uGC-UCGGCUGUAUGUgaaucacgCGCGCGCGACg -3' miRNA: 3'- -UGcAGCUGACGUGCG--------GCGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 53686 | 0.68 | 0.380055 |
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Target: 5'- uCGUCGACggGCACGgCaaGUGCGCuGGCg -3' miRNA: 3'- uGCAGCUGa-CGUGCgG--CGCGCG-CUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 54767 | 0.66 | 0.490552 |
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Target: 5'- cACGUCaccgcaaccGCUcGCGCGCCGUcuucgccGCGUGACa -3' miRNA: 3'- -UGCAGc--------UGA-CGUGCGGCG-------CGCGCUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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