Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24943 | 3' | -60.1 | NC_005284.1 | + | 9238 | 0.71 | 0.218016 |
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Target: 5'- gGCGcUGAC-GCGCGCgGCGgGCGGCa -3' miRNA: 3'- -UGCaGCUGaCGUGCGgCGCgCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 8977 | 0.71 | 0.223636 |
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Target: 5'- cGCGUCGAgcggUUGCcgugGUCGCGCGCGAUg -3' miRNA: 3'- -UGCAGCU----GACGug--CGGCGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 8877 | 0.75 | 0.125425 |
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Target: 5'- aACGaCGuGCUGCGCaUCGCGCGCGACa -3' miRNA: 3'- -UGCaGC-UGACGUGcGGCGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 8802 | 0.73 | 0.172699 |
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Target: 5'- cGCGgaUCGaACUGCGCGCCGCgucgaGCGCGcGCa -3' miRNA: 3'- -UGC--AGC-UGACGUGCGGCG-----CGCGC-UGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 8616 | 0.71 | 0.239426 |
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Target: 5'- uCGUCGACgGCcggcucguauuucaAUGCgGUGCGCGACg -3' miRNA: 3'- uGCAGCUGaCG--------------UGCGgCGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 7540 | 0.68 | 0.370709 |
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Target: 5'- aACGaCGAagggcaaaacauuCUGCGUGCCGCGCguGCGACg -3' miRNA: 3'- -UGCaGCU-------------GACGUGCGGCGCG--CGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 6531 | 0.71 | 0.229378 |
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Target: 5'- -aGUCGACgaagGCGCuCagGCGCGCGACa -3' miRNA: 3'- ugCAGCUGa---CGUGcGg-CGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 4122 | 0.69 | 0.293826 |
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Target: 5'- cCGgaagCGAUgGCAaucCGCCGUGCGCGAUg -3' miRNA: 3'- uGCa---GCUGaCGU---GCGGCGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 956 | 0.67 | 0.406335 |
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Target: 5'- gUGcCGAggUGUACGCCG-GCGCGACg -3' miRNA: 3'- uGCaGCUg-ACGUGCGGCgCGCGCUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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