Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24943 | 3' | -60.1 | NC_005284.1 | + | 43366 | 0.67 | 0.415345 |
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Target: 5'- -aGUCGAUacaGCAgGCCGUcCGCGACc -3' miRNA: 3'- ugCAGCUGa--CGUgCGGCGcGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 9327 | 0.67 | 0.406335 |
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Target: 5'- aGCGUaucCGuGCcGCcCGCCGCGCGCGuCa -3' miRNA: 3'- -UGCA---GC-UGaCGuGCGGCGCGCGCuGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 21646 | 0.67 | 0.388688 |
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Target: 5'- cGCGUCGA----ACGCC-CGCGCGGCc -3' miRNA: 3'- -UGCAGCUgacgUGCGGcGCGCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 33559 | 0.68 | 0.363179 |
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Target: 5'- uAUGUCGGCaGCAgCGCCGgccgacgGCGCGugUa -3' miRNA: 3'- -UGCAGCUGaCGU-GCGGCg------CGCGCugA- -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 35365 | 0.68 | 0.346829 |
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Target: 5'- cGCGUCGACUGUAgCGCaugGaCGC-CGACa -3' miRNA: 3'- -UGCAGCUGACGU-GCGg--C-GCGcGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 45652 | 0.68 | 0.346026 |
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Target: 5'- gGCGUCGAUagccgagUGCGCGggcgaCGCGCcacGCGGCg -3' miRNA: 3'- -UGCAGCUG-------ACGUGCg----GCGCG---CGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 15422 | 0.68 | 0.338854 |
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Target: 5'- --aUCGGcCUGCACGCCGa--GCGACa -3' miRNA: 3'- ugcAGCU-GACGUGCGGCgcgCGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 21755 | 0.68 | 0.338854 |
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Target: 5'- -gGUCGACgGCGgccacCGCCGaCGCgGCGGCa -3' miRNA: 3'- ugCAGCUGaCGU-----GCGGC-GCG-CGCUGa -5' |
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| 24943 | 3' | -60.1 | NC_005284.1 | + | 54767 | 0.66 | 0.490552 |
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Target: 5'- cACGUCaccgcaaccGCUcGCGCGCCGUcuucgccGCGUGACa -3' miRNA: 3'- -UGCAGc--------UGA-CGUGCGGCG-------CGCGCUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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