Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24943 | 5' | -53.3 | NC_005284.1 | + | 20596 | 0.66 | 0.824157 |
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Target: 5'- -----gCGCCGUACGGGUGAgCGgcggCAa -3' miRNA: 3'- agucaaGCGGCGUGUCCACU-GCaa--GU- -5' |
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| 24943 | 5' | -53.3 | NC_005284.1 | + | 53351 | 0.66 | 0.805191 |
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Target: 5'- gUCGcUUCGCCaGCGCA-GUGACGUa-- -3' miRNA: 3'- -AGUcAAGCGG-CGUGUcCACUGCAagu -5' |
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| 24943 | 5' | -53.3 | NC_005284.1 | + | 16864 | 0.67 | 0.754708 |
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Target: 5'- gCGGgUCGUaUGCGCAGGUGcuggccgguGCGUUCGa -3' miRNA: 3'- aGUCaAGCG-GCGUGUCCAC---------UGCAAGU- -5' |
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| 24943 | 5' | -53.3 | NC_005284.1 | + | 26528 | 0.67 | 0.751561 |
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Target: 5'- gCAGUUCGCCGCGCcgcucccguuuugGGCGggCAg -3' miRNA: 3'- aGUCAAGCGGCGUGucca---------CUGCaaGU- -5' |
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| 24943 | 5' | -53.3 | NC_005284.1 | + | 48828 | 0.68 | 0.711932 |
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Target: 5'- gCGGcgcaCGCCGCGCGGGuUGAUGgUUCu -3' miRNA: 3'- aGUCaa--GCGGCGUGUCC-ACUGC-AAGu -5' |
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| 24943 | 5' | -53.3 | NC_005284.1 | + | 7989 | 0.69 | 0.667861 |
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Target: 5'- -aAGUUCGCgCGCGCGaGUGACGa--- -3' miRNA: 3'- agUCAAGCG-GCGUGUcCACUGCaagu -5' |
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| 24943 | 5' | -53.3 | NC_005284.1 | + | 21552 | 0.69 | 0.667861 |
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Target: 5'- aUCGGcUUGCCGacgaGCAGGccgcgcgGGCGUUCGa -3' miRNA: 3'- -AGUCaAGCGGCg---UGUCCa------CUGCAAGU- -5' |
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| 24943 | 5' | -53.3 | NC_005284.1 | + | 53500 | 0.74 | 0.349454 |
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Target: 5'- cCGGUUCGCCGCGCGGGgcuuuUUCAu -3' miRNA: 3'- aGUCAAGCGGCGUGUCCacugcAAGU- -5' |
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| 24943 | 5' | -53.3 | NC_005284.1 | + | 30163 | 1.09 | 0.00156 |
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Target: 5'- gUCAGUUCGCCGCACAGGUGACGUUCAc -3' miRNA: 3'- -AGUCAAGCGGCGUGUCCACUGCAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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