Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24945 | 3' | -53.8 | NC_005284.1 | + | 12129 | 0.69 | 0.61993 |
Target: 5'- aUGCCGucggcGCGCUCG-GUGGUgcggCAAGCGc -3' miRNA: 3'- cGCGGU-----CGCGAGUgCAUCAa---GUUCGC- -5' |
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24945 | 3' | -53.8 | NC_005284.1 | + | 9518 | 0.68 | 0.686558 |
Target: 5'- gGCGCCcggcgAGCGCUCGuCG-AGgagacgaUCAAGCa -3' miRNA: 3'- -CGCGG-----UCGCGAGU-GCaUCa------AGUUCGc -5' |
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24945 | 3' | -53.8 | NC_005284.1 | + | 31682 | 1.1 | 0.001164 |
Target: 5'- uGCGCCAGCGCUCACGUAGUUCAAGCGc -3' miRNA: 3'- -CGCGGUCGCGAGUGCAUCAAGUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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