Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24947 | 3' | -53.3 | NC_005284.1 | + | 42269 | 0.66 | 0.831866 |
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Target: 5'- cGAGUgUGCGCCggucgUCGAggUCGUCagGAGCu -3' miRNA: 3'- -CUCA-ACGCGG-----AGCUgaAGUAG--CUCGc -5' |
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| 24947 | 3' | -53.3 | NC_005284.1 | + | 6608 | 0.66 | 0.822649 |
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Target: 5'- ----cGCGCCUgagCGcCUUCGUCGAcuGCGa -3' miRNA: 3'- cucaaCGCGGA---GCuGAAGUAGCU--CGC- -5' |
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| 24947 | 3' | -53.3 | NC_005284.1 | + | 9512 | 0.66 | 0.803605 |
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Target: 5'- uGGGUcgGCGCC-CGGCgagcgcUCGUCGAGg- -3' miRNA: 3'- -CUCAa-CGCGGaGCUGa-----AGUAGCUCgc -5' |
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| 24947 | 3' | -53.3 | NC_005284.1 | + | 9006 | 0.66 | 0.787833 |
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Target: 5'- --uUUGCGauccgcugauacagCUCGGCUgucgCGUCGAGCGg -3' miRNA: 3'- cucAACGCg-------------GAGCUGAa---GUAGCUCGC- -5' |
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| 24947 | 3' | -53.3 | NC_005284.1 | + | 45105 | 0.67 | 0.736005 |
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Target: 5'- ----aGCGCCgUUGACUgcugcgacgcugcgaUCGUCGAGCu -3' miRNA: 3'- cucaaCGCGG-AGCUGA---------------AGUAGCUCGc -5' |
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| 24947 | 3' | -53.3 | NC_005284.1 | + | 14579 | 0.69 | 0.610099 |
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Target: 5'- -cGUUGCGCUuggCGAUcUCGUCGGcGCGa -3' miRNA: 3'- cuCAACGCGGa--GCUGaAGUAGCU-CGC- -5' |
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| 24947 | 3' | -53.3 | NC_005284.1 | + | 36073 | 0.7 | 0.59895 |
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Target: 5'- cGAGUUGCGagagCUUGAUUUCGUcCGGuGCGg -3' miRNA: 3'- -CUCAACGCg---GAGCUGAAGUA-GCU-CGC- -5' |
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| 24947 | 3' | -53.3 | NC_005284.1 | + | 54440 | 0.71 | 0.500942 |
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Target: 5'- ----gGUGCUUgcaCGGCUUCAUCGGGCGu -3' miRNA: 3'- cucaaCGCGGA---GCUGAAGUAGCUCGC- -5' |
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| 24947 | 3' | -53.3 | NC_005284.1 | + | 33834 | 1.08 | 0.001744 |
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Target: 5'- aGAGUUGCGCCUCGACUUCAUCGAGCGc -3' miRNA: 3'- -CUCAACGCGGAGCUGAAGUAGCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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