Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24947 | 5' | -52 | NC_005284.1 | + | 21348 | 0.66 | 0.911294 |
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Target: 5'- uGUCGcgCGaucaaCGAGUCG-GACAACggUCGGc -3' miRNA: 3'- -CAGCa-GCa----GCUCAGCuCUGUUG--AGCC- -5' |
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| 24947 | 5' | -52 | NC_005284.1 | + | 48899 | 0.67 | 0.859728 |
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Target: 5'- aGUCGagccacgCGUCGAcgggaaccaGUCG-GGCAGCUCGa -3' miRNA: 3'- -CAGCa------GCAGCU---------CAGCuCUGUUGAGCc -5' |
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| 24947 | 5' | -52 | NC_005284.1 | + | 32797 | 0.68 | 0.824894 |
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Target: 5'- -gCGUCGgccugcgCGGGUugCGGGGCGGCgUCGGu -3' miRNA: 3'- caGCAGCa------GCUCA--GCUCUGUUG-AGCC- -5' |
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| 24947 | 5' | -52 | NC_005284.1 | + | 7150 | 0.68 | 0.824894 |
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Target: 5'- -gUGUCGaCGAGUUGccGGACGACuauUCGGa -3' miRNA: 3'- caGCAGCaGCUCAGC--UCUGUUG---AGCC- -5' |
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| 24947 | 5' | -52 | NC_005284.1 | + | 193 | 0.7 | 0.692825 |
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Target: 5'- aUUGUCGgCGAGgcgCGGGGCAugUgGGa -3' miRNA: 3'- cAGCAGCaGCUCa--GCUCUGUugAgCC- -5' |
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| 24947 | 5' | -52 | NC_005284.1 | + | 27172 | 0.71 | 0.660026 |
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Target: 5'- cGUCGUUGcUCGAGUaUGGGGCGccGCUaCGGa -3' miRNA: 3'- -CAGCAGC-AGCUCA-GCUCUGU--UGA-GCC- -5' |
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| 24947 | 5' | -52 | NC_005284.1 | + | 51149 | 0.75 | 0.456994 |
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Target: 5'- -cCGUCGUCGAGcUCGAcacccucgagcgGACAGC-CGGg -3' miRNA: 3'- caGCAGCAGCUC-AGCU------------CUGUUGaGCC- -5' |
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| 24947 | 5' | -52 | NC_005284.1 | + | 33870 | 1.1 | 0.00239 |
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Target: 5'- uGUCGUCGUCGAGUCGAGACAACUCGGu -3' miRNA: 3'- -CAGCAGCAGCUCAGCUCUGUUGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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