Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 24948 | 3' | -57.5 | NC_005284.1 | + | 31518 | 0.66 | 0.54136 |
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Target: 5'- gGGCCCggcgcgccgcagcgGCgcaCGUCGCCCGCGaacugGCGCa- -3' miRNA: 3'- aCUGGG--------------UGa--GUAGCGGGUGC-----UGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 6334 | 0.66 | 0.57684 |
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Target: 5'- --uCCCACUCAaaaagcgaacguUCGCCCACacacuGGCGUg- -3' miRNA: 3'- acuGGGUGAGU------------AGCGGGUG-----CUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 10705 | 0.66 | 0.587702 |
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Target: 5'- cGcCgCCGCUCGUCGCCgaCACGAaGCg- -3' miRNA: 3'- aCuG-GGUGAGUAGCGG--GUGCUgCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 8989 | 0.66 | 0.57684 |
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Target: 5'- cGGCCCGCUUGUCa-CCACGAUcaGCa- -3' miRNA: 3'- aCUGGGUGAGUAGcgGGUGCUG--CGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 54717 | 0.66 | 0.57684 |
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Target: 5'- gUGGCCCGuccgUCGUUGCCCGCccggcgGAUGCg- -3' miRNA: 3'- -ACUGGGUg---AGUAGCGGGUG------CUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 10937 | 0.66 | 0.55526 |
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Target: 5'- aGGCCCGCauggccggccUCGUCGCCggacgaGCGgACGCa- -3' miRNA: 3'- aCUGGGUG----------AGUAGCGGg-----UGC-UGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 31206 | 0.66 | 0.533923 |
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Target: 5'- cGAUCuCGCguagCAUCG-CCGCGACGUUg -3' miRNA: 3'- aCUGG-GUGa---GUAGCgGGUGCUGCGAa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 46785 | 0.66 | 0.544558 |
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Target: 5'- -uGCUCAUuggCGUCGCCCaAUGGCGCg- -3' miRNA: 3'- acUGGGUGa--GUAGCGGG-UGCUGCGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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