Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24948 | 3' | -57.5 | NC_005284.1 | + | 16104 | 0.7 | 0.343447 |
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Target: 5'- -cGCCgCACUCGUUGCCguUGGCGCg- -3' miRNA: 3'- acUGG-GUGAGUAGCGGguGCUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 21997 | 0.7 | 0.327274 |
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Target: 5'- cGAUCuCGCgcugaUCGUCGCCgGCGACGCc- -3' miRNA: 3'- aCUGG-GUG-----AGUAGCGGgUGCUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 10491 | 0.7 | 0.327274 |
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Target: 5'- cGACUgCGCgUCAUcggcCGCCCGCGACGCc- -3' miRNA: 3'- aCUGG-GUG-AGUA----GCGGGUGCUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 33957 | 0.71 | 0.296642 |
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Target: 5'- -cGCCCGCUCGUCgGCCgGCGcUGCUg -3' miRNA: 3'- acUGGGUGAGUAG-CGGgUGCuGCGAa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 48811 | 0.71 | 0.282181 |
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Target: 5'- -uGCCCgACUgGUUGCCCGuCGACGCg- -3' miRNA: 3'- acUGGG-UGAgUAGCGGGU-GCUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 50351 | 0.72 | 0.275163 |
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Target: 5'- uUGAUCCGCUUcacgAUCGCCUucUGACGCUUc -3' miRNA: 3'- -ACUGGGUGAG----UAGCGGGu-GCUGCGAA- -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 10760 | 0.77 | 0.113451 |
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Target: 5'- aGGCCCGCcgugUCGaucgCGCCCGCGGCGCg- -3' miRNA: 3'- aCUGGGUG----AGUa---GCGGGUGCUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 34431 | 1.05 | 0.001056 |
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Target: 5'- aUGACCCACUCAUCGCCCACGACGCUUc -3' miRNA: 3'- -ACUGGGUGAGUAGCGGGUGCUGCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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