Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24948 | 3' | -57.5 | NC_005284.1 | + | 34671 | 0.68 | 0.423117 |
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Target: 5'- cGuACUCACUCA-CGUUgACGACGCUg -3' miRNA: 3'- aC-UGGGUGAGUaGCGGgUGCUGCGAa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 38979 | 0.68 | 0.452032 |
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Target: 5'- cUGAUCCGCgggCugcgCGCUgGCGACGCa- -3' miRNA: 3'- -ACUGGGUGa--Gua--GCGGgUGCUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 46110 | 0.69 | 0.404468 |
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Target: 5'- aGGCCUGCUCGaCGCCCgGCGACa--- -3' miRNA: 3'- aCUGGGUGAGUaGCGGG-UGCUGcgaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 46785 | 0.66 | 0.544558 |
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Target: 5'- -uGCUCAUuggCGUCGCCCaAUGGCGCg- -3' miRNA: 3'- acUGGGUGa--GUAGCGGG-UGCUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 48811 | 0.71 | 0.282181 |
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Target: 5'- -uGCCCgACUgGUUGCCCGuCGACGCg- -3' miRNA: 3'- acUGGG-UGAgUAGCGGGU-GCUGCGaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 49976 | 0.69 | 0.404468 |
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Target: 5'- cGAUCUGCUCAaucgUCaGCCCGCGGCGg-- -3' miRNA: 3'- aCUGGGUGAGU----AG-CGGGUGCUGCgaa -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 50351 | 0.72 | 0.275163 |
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Target: 5'- uUGAUCCGCUUcacgAUCGCCUucUGACGCUUc -3' miRNA: 3'- -ACUGGGUGAG----UAGCGGGu-GCUGCGAA- -5' |
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| 24948 | 3' | -57.5 | NC_005284.1 | + | 54717 | 0.66 | 0.57684 |
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Target: 5'- gUGGCCCGuccgUCGUUGCCCGCccggcgGAUGCg- -3' miRNA: 3'- -ACUGGGUg---AGUAGCGGGUG------CUGCGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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