Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24949 | 5' | -51 | NC_005284.1 | + | 49519 | 0.73 | 0.518075 |
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Target: 5'- aGAGUgGCCGG-UCGAggucGCCGGAUGCg -3' miRNA: 3'- aCUUA-CGGCUaAGCUa---CGGCUUGCGa -5' |
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| 24949 | 5' | -51 | NC_005284.1 | + | 18057 | 0.74 | 0.475352 |
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Target: 5'- ----cGCCGAUcCGGUGgCGGGCGCUg -3' miRNA: 3'- acuuaCGGCUAaGCUACgGCUUGCGA- -5' |
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| 24949 | 5' | -51 | NC_005284.1 | + | 38581 | 0.75 | 0.454656 |
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Target: 5'- aGAcgGCCG-UUCG--GCCGAGCGCa -3' miRNA: 3'- aCUuaCGGCuAAGCuaCGGCUUGCGa -5' |
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| 24949 | 5' | -51 | NC_005284.1 | + | 35408 | 0.75 | 0.424557 |
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Target: 5'- gGAAU-CCGAcgUCGAUGCCGAAaGCUa -3' miRNA: 3'- aCUUAcGGCUa-AGCUACGGCUUgCGA- -5' |
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| 24949 | 5' | -51 | NC_005284.1 | + | 27829 | 0.79 | 0.278669 |
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Target: 5'- ----gGCCGAUUCGAUGCCaGACGUUu -3' miRNA: 3'- acuuaCGGCUAAGCUACGGcUUGCGA- -5' |
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| 24949 | 5' | -51 | NC_005284.1 | + | 43521 | 0.79 | 0.278669 |
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Target: 5'- cGGcUGCUguGAUUCGAUGCCGAuaGCGCg -3' miRNA: 3'- aCUuACGG--CUAAGCUACGGCU--UGCGa -5' |
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| 24949 | 5' | -51 | NC_005284.1 | + | 35361 | 1.08 | 0.003057 |
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Target: 5'- aUGAAUGCCGAUUCGAUGCCGAACGCUu -3' miRNA: 3'- -ACUUACGGCUAAGCUACGGCUUGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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