Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24950 | 5' | -52.6 | NC_005284.1 | + | 33395 | 0.69 | 0.662676 |
Target: 5'- -gGCUCGCcaucACCCgauUCGAGauaGAAGCa -3' miRNA: 3'- aaCGAGCGu---UGGGa--AGCUCg--CUUUGc -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 35490 | 1.07 | 0.00215 |
Target: 5'- uUUGCUCGCAACCCUUCGAGCGAAACGa -3' miRNA: 3'- -AACGAGCGUUGGGAAGCUCGCUUUGC- -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 37637 | 0.66 | 0.802518 |
Target: 5'- cUGCUCGCcguGCCgUUCGuGUGGcuGGCa -3' miRNA: 3'- aACGAGCGu--UGGgAAGCuCGCU--UUGc -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 37797 | 0.71 | 0.517141 |
Target: 5'- ----gCGCGGCCUUUCG-GCGggGCGg -3' miRNA: 3'- aacgaGCGUUGGGAAGCuCGCuuUGC- -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 45649 | 0.7 | 0.594762 |
Target: 5'- -gGCUgGCGGCUCgUCGGugcGCGAGGCGg -3' miRNA: 3'- aaCGAgCGUUGGGaAGCU---CGCUUUGC- -5' |
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24950 | 5' | -52.6 | NC_005284.1 | + | 51140 | 0.68 | 0.718528 |
Target: 5'- -aGCUCGacACCC-UCGAGCGGAcaGCc -3' miRNA: 3'- aaCGAGCguUGGGaAGCUCGCUU--UGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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