Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24950 | 5' | -52.6 | NC_005284.1 | + | 21347 | 0.68 | 0.718528 |
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Target: 5'- -gGCgCGCGGCCCgaugUUGuaGGCGAAGCu -3' miRNA: 3'- aaCGaGCGUUGGGa---AGC--UCGCUUUGc -5' |
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| 24950 | 5' | -52.6 | NC_005284.1 | + | 1705 | 0.68 | 0.718528 |
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Target: 5'- -aGCUUGCGcGCCaugUCGAGCGcGAGCa -3' miRNA: 3'- aaCGAGCGU-UGGga-AGCUCGC-UUUGc -5' |
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| 24950 | 5' | -52.6 | NC_005284.1 | + | 14850 | 0.67 | 0.761649 |
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Target: 5'- gUUGCUgGCcACCgaagUCGAGCGGcGACGa -3' miRNA: 3'- -AACGAgCGuUGGga--AGCUCGCU-UUGC- -5' |
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| 24950 | 5' | -52.6 | NC_005284.1 | + | 12536 | 0.67 | 0.789531 |
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Target: 5'- -gGCUCGCGggguucgacgauccGCCC-UCGAuCGAAACu -3' miRNA: 3'- aaCGAGCGU--------------UGGGaAGCUcGCUUUGc -5' |
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| 24950 | 5' | -52.6 | NC_005284.1 | + | 37637 | 0.66 | 0.802518 |
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Target: 5'- cUGCUCGCcguGCCgUUCGuGUGGcuGGCa -3' miRNA: 3'- aACGAGCGu--UGGgAAGCuCGCU--UUGc -5' |
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| 24950 | 5' | -52.6 | NC_005284.1 | + | 5878 | 0.79 | 0.191247 |
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Target: 5'- gUGCaagCGCAACC--UCGAGCGAAACGa -3' miRNA: 3'- aACGa--GCGUUGGgaAGCUCGCUUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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