Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24951 | 3' | -62.7 | NC_005284.1 | + | 17881 | 0.67 | 0.285475 |
Target: 5'- cGGCCGCCggAUCGgCGGGCGcaUCCGCg-- -3' miRNA: 3'- -UUGGCGG--UGGCgGCUCGC--AGGCGaga -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 12820 | 0.67 | 0.292441 |
Target: 5'- --aCGCCGCCGCUucGCGcCCGCg-- -3' miRNA: 3'- uugGCGGUGGCGGcuCGCaGGCGaga -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 18561 | 0.67 | 0.299539 |
Target: 5'- -uCCGCCGCCGUCGGuCGUgUCGCUg- -3' miRNA: 3'- uuGGCGGUGGCGGCUcGCA-GGCGAga -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 33776 | 0.66 | 0.304587 |
Target: 5'- aGugCGCCGgCGCCGAcuucguagucgaucGCG-CCGUUCg -3' miRNA: 3'- -UugGCGGUgGCGGCU--------------CGCaGGCGAGa -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 40176 | 0.66 | 0.314134 |
Target: 5'- gAACCGCCACgugCGUCGcgaccaacuccGGCGUCCucugGCUCc -3' miRNA: 3'- -UUGGCGGUG---GCGGC-----------UCGCAGG----CGAGa -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 37077 | 0.66 | 0.314134 |
Target: 5'- cGACCGCCAUcgacgCGCCGgcgagauucagGGCGUCgCGCa-- -3' miRNA: 3'- -UUGGCGGUG-----GCGGC-----------UCGCAG-GCGaga -5' |
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24951 | 3' | -62.7 | NC_005284.1 | + | 36570 | 1.04 | 0.000416 |
Target: 5'- cAACCGCCACCGCCGAGCGUCCGCUCUu -3' miRNA: 3'- -UUGGCGGUGGCGGCUCGCAGGCGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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